TRIM9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM9 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 10786-1-AP

Synonyme

KIAA0282, RING finger protein 91, RNF91, SPRING, TRIM9, tripartite motif containing 9

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Galerie der Validierungsdaten

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  WB analysis using 10786-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10786-1-AP (TRIM9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10786-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10786-1-AP (TRIM9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10786-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10786-1-AP (TRIM9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10786-1-AP

  IP result of anti-TRIM9 (IP:10786-1-AP, 4ug; Detection:10786-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of rat brain using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10786-1-AP (TRIM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10786-1-AP (TRIM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 10786-1-AP (TRIM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 10786-1-AP (TRIM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10786-1-AP (TRIM9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10786-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10786-1-AP (TRIM9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Prostatakarzinomgewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

10786-1-AP bindet in WB, IP, IHC, ELISA TRIM9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM9 fusion protein Ag1238
• Vollständiger Name: tripartite motif-containing 9
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 79 kDa, 90 kDa, 61 kDa
• GenBank-Zugangsnummer: BC013414
• Gene symbol: TRIM9
• Gene ID (NCBI): 114088
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM9(E3 ubiquitin-protein ligase TRIM9) is also named as RNF91 and belongs to the TRIM/RBCC family. TRIM9 protein is a brain-specific E3 ubiquitin ligase. Importantly, TRIM9 is present in LBs found in DLB and PD, suggesting that TRIM9 not only plays roles in the regulation of neuronal functions, but also participates in the formation or breakdown of abnormal inclusions through its ligase activity and besides the striatum and hippocampus, the highest expression of TRIM9 protein is seen in the frontal, temporal and occipital cortices through the western blot (PMID:20085810). It has been reported that TRIM9 is highly expressed in the cerebral cortex in mouse and human brains and that TRIM9 is decreased in damaged brains in patients who have Parkinson disease and dementia with Lewy bodies(PMID:22337885). It has 3 isoforms with molecular mass of 79, 90 and 61 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Res; PMID: 26915459; Authors - Yunfei Qin ; KD Validated
human, mouse, rat	WB	Neurobiol Dis; TRIM9, a novel brain-specific E3 ubiquitin ligase, is repressed in the brain of Parkinson's disease and dementia with Lewy bodies.; Authors - Tanji Kunikazu K
rat	WB,IP	Mol Med Rep; Knockdown of TRIM9 attenuates irinotecan‑induced intestinal mucositis in IEC‑6 cells by regulating DUSP6 expression via the P38 pathway.; Authors - Wenjun Zhao ; KD Validated
human	IHC	Springerplus; Promoter methylation of TRIM9 as a marker for detection of circulating tumor DNA in breast cancer patients.; Authors - Chieko Mishima
mouse	WB	Elife; A twin UGUA motif directs the balance between gene isoforms through CFIm and the mTORC1 signaling pathway; Authors - R Samuel Herron
mouse	WB	Mol Biol Cell; The E3 ubiquitin ligase TRIM9 regulates synaptic function and actin dynamics in response to netrin-1; Authors - Laura E McCormick ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Echo (Verified Customer) (03-20-2024)

The quality was good. Saw two bands for western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

TRIM9 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:10786-1-AP)