TRIM69 Polyklonaler Antikörper

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• KD/KO Validated

TRIM69 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12951-1-AP

Synonyme

HSD 34, HSD34, RING finger protein 36, RNF36, Trif, TRIM69, tripartite motif containing 69

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 12951-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12951-1-AP (TRIM69 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12951-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12951-1-AP (TRIM69 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12951-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12951-1-AP (TRIM69 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12951-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA TRIM69 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM69 fusion protein Ag4074
• Vollständiger Name: tripartite motif-containing 69
• Berechnetes Molekulargewicht: 500 aa, 57 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC033314
• Gene symbol: TRIM69
• Gene ID (NCBI): 140691
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

E3 ubiquitin-protein ligase TRIM69 (TRIM69) plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis Indiana virus (PubMed:31375575, PubMed:31578292). It plays also a role in cataract formation together with TP53 (PubMed:30844644). Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination (PubMed:30844644). It has 4 isoforms with molecular weights of 57, 39, 34, and 32 kDa.

Publikationen

Species	Application	Title
Human,monkey	WB	Front Immunol; DExD/H-Box Helicase 36 Signaling via Myeloid Differentiation Primary Response Gene 88 Contributes to NF-κB Activation to Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection.; Authors - Huiyuan Jing
human	WB	Cell Physiol Biochem; Radioprotective Effects of Heat-Killed Mycobacterium Tuberculosis in Cultured Cells and Radiosensitive Tissues.; Authors - Yuanyuan Chen
human	WB	J Virol; Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State.; Authors - Tonya Kueck
sheep	WB	Mediators Inflamm; Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.; Authors - Zhongjia Jiang
mouse	IF, WB	J Biomed Res; The testis-specifically expressed gene Trim69 is not essential for fertility in mice.; Authors - Xi He ; KO Validated
human	WB,IHC,IF,IP,CoIP	Oncogene; TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD; Authors - Linqing Sun ; KD Validated