TRIM6 Polyklonaler Antikörper

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• KD/KO Validated

TRIM6 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11953-1-AP

Synonyme

RING finger protein 89, RNF89, TRIM6, tripartite motif containing 6

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Galerie der Validierungsdaten

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  WB analysis using 11953-1-AP

  HepG2 cell lysates were subjected to SDS PAGE followed by western blot with 11953-1-AP (TRIM6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11953-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11953-1-AP (TRIM6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11953-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11953-1-AP (TRIM6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11953-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11953-1-AP (TRIM6 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, humanes Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

11953-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA TRIM6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM6 fusion protein Ag2556
• Vollständiger Name: tripartite motif-containing 6
• Berechnetes Molekulargewicht: 488 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC065575
• Gene symbol: TRIM6
• Gene ID (NCBI): 117854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IP	Oxid Med Cell Longev; TRIM6 Reduces Ferroptosis and Chemosensitivity by Targeting SLC1A5 in Lung Cancer; Authors - Ying Zhang
human	IHC	Front Oncol; Tripartite Motif Protein 6 Promotes Colorectal Cancer Cell Migration and Metastasis via SOCS2-STAT3 Signaling; Authors - Hongjian Zhao
human	WB,IHC,CoIP	J Exp Clin Cancer Res; TRIM6 promotes colorectal cancer cells proliferation and response to thiostrepton by TIS21/FoxM1.; Authors - Shuier Zheng ; KD Validated
human	WB,IHC	J Chem Neuroanat; Silencing TRIM6 inhibits the growth and angiogenesis of gliomas by regulating VEGFA; Authors - Xin Liu
human	WB,IF,IP	J Med Virol; TRIM6 facilitates SARS-CoV-2 proliferation by catalyzing the K29-typed ubiquitination of NP to enhance the ability to bind viral genomes; Authors - Jian Zhou ; KD Validated
human,mouse	WB,IHC,CoIP	Transl Oncol; TRIM6 promotes glioma malignant progression by enhancing FOXO3A ubiquitination and degradation; Authors - Jingpeng Guo ; KD Validated