TRIM5 Polyklonaler Antikörper

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• KD/KO Validated

TRIM5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11476-1-AP

Synonyme

RING finger protein 88, RNF88, TRIM5, TRIM5alpha, tripartite motif containing 5

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11476-1-AP

  WB result of TRIM5 antibody (11476-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM5 transfected HeLa cells.

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  WB analysis using 11476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11476-1-AP (TRIM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11476-1-AP (TRIM5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 11476-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 11476-1-AP (TRIM5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 11476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11476-1-AP (TRIM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 11476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11476-1-AP (TRIM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 11476-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11476-1-AP (TRIM5 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MKN-45-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11476-1-AP bindet in WB, IHC, ELISA TRIM5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM5 fusion protein Ag1973
• Vollständiger Name: tripartite motif-containing 5
• Berechnetes Molekulargewicht: 493 aa, 56 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 56 kDa
• GenBank-Zugangsnummer: BC021258
• Gene symbol: TRIM5
• Gene ID (NCBI): 85363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM5 belongs to the large tripartite motif protein family, members of which typically are composed of 3 zinc-binding domains, a RING, unique B-box type 1 and B-box type 2 domains, followed by a coiled-coil (CC) region. TRIM proteins use homomultimerization to identify specific cell compartments. TRIM5 promotes innate immune signaling and that this activity is amplified by retroviral infection and interaction with the capsid lattice. TRIM5 has 6 variants termed alpha, beta, gamma, delta, epsilon, and iota with the MW of 56 kDa, 46 kDa, 40 kDa, 37 kDa, 31 kDa and 29 kDa. TRIM5 can form homodimers (~70 kDa) and homotrimers (90-160 kDa).