TRIM44 Polyklonaler Antikörper

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• KD/KO Validated

TRIM44 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 11511-1-AP

Synonyme

DIPB, HSA249128, MC7, Protein DIPB, TRIM44, tripartite motif containing 44

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 11511-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11511-1-AP (TRIM44 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11511-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11511-1-AP (TRIM44 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11511-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11511-1-AP (TRIM44 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, humanes Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 16 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Produktinformation

11511-1-AP bindet in WB, IP, IF, IHC, ELISA TRIM44 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM44 fusion protein Ag2070
• Vollständiger Name: tripartite motif-containing 44
• Berechnetes Molekulargewicht: 344 aa, 38 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC024031
• Gene symbol: TRIM44
• Gene ID (NCBI): 54765
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM44 is one of the family member of TRIM protein, which contains an N-terminal ubiquitin hydrolase-type zinc-finger domain, followed by a coiled-coil domain, a zinc-finger B-box homology domain, and a second coiled-coil domain near the C-terminus. Members of the TRIM protein family are involved in various cellular processes, such as cell proliferation, oncogenesis and antiviral defense. This is a rabbit polyantibody raised against full length of human TRIM44.

Publikationen

Species	Application	Title
human	IHC	Gastroenterology; A 4-gene signature predicts survival of patients with resected adenocarcinoma of the esophagus, junction, and gastric cardia.; Authors - Peters Christopher J CJ
human	WB,IF	Autophagy; TRIM44 links the UPS to SQSTM1/p62-dependent aggrephagy and removing misfolded proteins.; Authors - Lin Lyu ; KO Validated
human	IP,WB,IF	Oncogene; TRIM44 mediated p62 deubiquitination enhances DNA damage repair by increasing nuclear FLNA and 53BP1 expression.; Authors - Lin Lyu ; KO Validated
human	WB	J Immunol; Novel function of Trim44 promotes an antiviral response by stabilizing VISA.; Authors - Yang Bo B
rat	WB,IHC,IF	Dis Model Mech; Cardiac-specific Trim44 knockout in rat attenuates isoproterenol-induced cardiac remodeling via inhibition of AKT/mTOR pathway.; Authors - Xiao-Yu Jiang
human	WB,IHC	Cancer Sci; TRIM44 promotes human esophageal cancer progression via the AKT/mTOR pathway.; Authors - Dian Xiong