TRIM38 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM38 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13405-1-AP

Synonyme

TRIM, RORET, RNF15, RING finger protein 15, EC:2.3.2.27

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Galerie der Validierungsdaten

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  WB analysis of human liver using 13405-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 13405-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13405-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 13405-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13405-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13405-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 13405-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 13405-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed L02 cells using 13405-1-AP (TRIM38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, HEK-293-Zellen, humanes Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

13405-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA TRIM38 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM38 fusion protein Ag3840
• Vollständiger Name: tripartite motif-containing 38
• Berechnetes Molekulargewicht: 465 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC026930
• Gene symbol: TRIM38
• Gene ID (NCBI): 10475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The proinflammatory cytokines TNFα and IL-1β play central roles in many diseases, including infectious diseases, autoimmunity, and cancers. TRIM38 belongs to the TRIM protein family that is involved in various biological processes, including cell proliferation and apoptosis, innate immunity, and inflammatory response. It has been shown that TRIM38 acts as a suppressor in TOLL-like receptor (TLR)-mediated IFN-β induction by promoting degradation of TRAF6 and NAP1 through the ubiquitinproteasome system. (PMID:24434549)

Publikationen

Species	Application	Title
human	IHC	Aging (Albany NY); Tertiary lymphoid structure stratifies glioma into three distinct tumor subtypes.; Authors - Xingwang Zhou
mouse	WB	Int Immunopharmacol; TRIM38 protects chondrocytes from IL-1β-induced apoptosis and degeneration via negatively modulating nuclear factor (NF)-κB signaling.; Authors - Shouye Hu ; KD Validated
mouse	WB,IHC	J Lipid Res; Tripartite motif 38 alleviates the pathological process of NAFLD/NASH by promoting TAB2 degradation; Authors - Xinxin Yao
mouse	WB,CoIP	Phytomedicine; Active fraction of Polyrhachis vicina (Rogers) inhibits osteoclastogenesis by targeting Trim38 mediated proteasomal degradation of TRAF6; Authors - Xiaoliang Feng