TRIM37 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM37 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13037-1-AP

Synonyme

RING-type E3 ubiquitin transferase TRIM37, POB1, MUL, KIAA0898, EC:2.3.2.27

---

Galerie der Validierungsdaten

• 

  WB analysis using 13037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13037-1-AP

  IP result of anti-TRIM37 (IP:13037-1-AP, 4ug; Detection:13037-1-AP 1:800) with mouse brain tissue lysate 1800 ug.

• 

  IHC staining of mouse testis using 13037-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13037-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13037-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 13037-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using TRIM37 antibody (13037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of U-87 MG using 13037-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using TRIM37 antibody (13037-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

13037-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TRIM37 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM37 fusion protein Ag3690
• Vollständiger Name: tripartite motif-containing 37
• Berechnetes Molekulargewicht: 964 aa, 108 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC036012
• Gene symbol: TRIM37
• Gene ID (NCBI): 4591
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM37 (tripartite motif containing 37) is a member of the tripartite motif (RING, B-Box, and coiled-coil domains) family . Its gene mutations result in mulibrey nanism, which is a rare, autosomal-recessive growth disorder of prenatal onset, with characteristic dysmorphic features, pericardial constriction, and hepatomegaly .TRIM37 is located on chromosome 17q23, encoding a protein with an estimated molecular weight of 130 kDa or 89kDa. (PMID: 28098873, PMID: 29940807, PMID: 14757854)

Publikationen

Species	Application	Title
human	IHC	Transl Oncol; TRIM37 promotes gallbladder cancer proliferation by activating the Wnt/β-catenin pathway via ubiquitination of Axin1; Authors - Ming Xu
human	WB,IHC,IP	Exp Cell Res; Aerobic glycolysis and tumor progression of hepatocellular carcinoma are mediated by ubiquitin of P53 K48-linked regulated by TRIM37; Authors - Yuzhen Ge ; KD Validated
human	WB,IP	Biochem Biophys Res Commun; Tripartite motif containing protein 37 involves in thrombin stimulated BV-2 microglial cell apoptosis and interleukin 1β release.; Authors - Chong Han ; KD Validated
human	WB	Mol Cancer Res; Downregulation of BASP1 promotes temozolomide resistance in gliomas via epigenetic activation of the FBXO32/NF-κB/MGMT axis; Authors - Xinyi Liao
mouse	WB,IF	Mol Med; TRIM37 exacerbates hepatic ischemia/reperfusion injury by facilitating IKKγ translocation; Authors - Hang Yang ; KD Validated
human,mouse	WB,IF	Cell Discov; The TRIM37 variants in Mulibrey nanism patients paralyze follicular helper T cell differentiation; Authors - Wangpeng Gu