TRIM22 Polyklonaler Antikörper

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• KD/KO Validated

TRIM22 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13744-1-AP

Synonyme

GPSTAF50, RING finger protein 94, RNF94, Staf 50, STAF50, TRIM22, tripartite motif containing 22

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Galerie der Validierungsdaten

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  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, Mausmilzgewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

13744-1-AP bindet in WB, IF, IHC, ELISA TRIM22 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM22 fusion protein Ag4683
• Vollständiger Name: tripartite motif-containing 22
• Berechnetes Molekulargewicht: 498 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC035582
• Gene symbol: TRIM22
• Gene ID (NCBI): 10346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines (PMID: 23818111). TRIM22 is involved in the host antiviral response, TRIM22 expression is modulated in response to several other viruses and viral antigens.TRIM22 expression is high in monocytes and early granulocytes but decreases in the lymphocyte and late granulocyte populations and is undetectable in erythroid cells (PMID: 32814880).

Publikationen

Species	Application	Title
human	WB	Nat Commun; Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.; Authors - Alice Mac Kain
human	WB,IHC	Oncotarget; TRIM22 confers poor prognosis and promotes epithelial-mesenchymal transition through regulation of AKT/GSK3β/β-catenin signaling in non-small cell lung cancer.; Authors - Li Liu ; KD Validated
human	WB,IHC,IF	Front Cell Infect Microbiol; Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.; Authors - Matthew Charman ; KD Validated
human	IHC	Immunobiology; Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations; Authors - Tiantian Cai
human	WB	Cell Biol Int; TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.; Authors - Liyin Li ; KD Validated
human	WB	Transl Cancer Res; FOXO3/TRIM22 axis abated the antitumor effect of gemcitabine in non-small cell lung cancer via autophagy induction.; Authors - Yang Wang ; KD Validated