TRIM15 Polyklonaler Antikörper

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• KD/KO Validated

TRIM15 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13623-1-AP

Synonyme

RING finger protein 93, RNF93, TRIM15, tripartite motif containing 15, Zinc finger protein 178, Zinc finger protein B7, ZNF178, ZNFB7

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 13623-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13623-1-AP (TRIM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13623-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13623-1-AP (TRIM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 13623-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13623-1-AP (TRIM15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13623-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13623-1-AP (TRIM15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

13623-1-AP bindet in WB, IHC, IP, ELISA, IF TRIM15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM15 fusion protein Ag4533
• Vollständiger Name: tripartite motif-containing 15
• Berechnetes Molekulargewicht: 465 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC038585
• Gene symbol: TRIM15
• Gene ID (NCBI): 89870
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM15 (Tripartite motif-containing protein 15) is also named as RNF93, ZNF178, ZNFB7 and belongs to the TRIM/RBCC family. It acts as an ubiquitin-protein ligase and contributes to the endogenous restriction of retroviruses in cells.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination.; Authors - Guixin Zhu ; KO Validated
human	WB,IP,IF	Biochim Biophys Acta Mol Basis Dis; Ubiquitin ligase TRIM15 promotes the progression of pancreatic cancer via the upregulation of the IGF2BP2-TLR4 axis; Authors - Hongkun Cai
human	IF	J Cell Sci; TRIM15 is a focal adhesion protein that regulates focal adhesion disassembly.; Authors - Pradeep D Uchil ; KD Validated
human	IHC	iScience; Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma; Authors - Qidong Cai
human	WB	J Virol; TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.; Authors - Uchil Pradeep D PD ; KD Validated
human	IHC	J Cancer; Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses.; Authors - Xiaoying Han