TRIM11 Polyklonaler Antikörper

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• KD/KO Validated

TRIM11 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10851-1-AP

Synonyme

BIA1, Protein BIA1, RING finger protein 92, RNF92, TRIM11, tripartite motif containing 11

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Galerie der Validierungsdaten

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  WB analysis using 10851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10851-1-AP (TRIM11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• CoIP: See 5 publications below

Produktinformation

10851-1-AP bindet in WB, IF, IHC, CoIP, ELISA TRIM11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM11 fusion protein Ag1308
• Vollständiger Name: tripartite motif-containing 11
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC011629
• Gene symbol: TRIM11
• Gene ID (NCBI): 81559
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM11(Tripartite motif-containing protein 11) is also named as RNF92 and Belongs to the TRIM/RBCC family. It is a member of the tripartite-motif-containing protein family and is known to destabilize humanin, an inhibitor of Alzheimer-like neuronal insults(PMID: 16904669). This protein has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
		Science; TRIM11 protects against tauopathies and is down-regulated in Alzheimer's disease; Authors - Zi-Yang Zhang
mouse	WB,IF,CoIP	J Hazard Mater; The proteasome-dependent degradation of ALKBH5 regulates ECM deposition in PM2.5 exposure-induced pulmonary fibrosis of mice.; Authors - Yaling Zhang
human	IHC,IF	Oncogene; TRIM11 is overexpressed in high-grade gliomas and promotes proliferation, invasion, migration and glial tumor growth.; Authors - Di K K
mouse	WB,IHC	FASEB J; The topological key lncRNA H2k2 from the ceRNA network promotes mesangial cell proliferation in diabetic nephropathy via the miR-449a/b/Trim11/Mek signaling pathway.; Authors - Wenyun Chen
human	IF,CoIP, WB	Int J Biol Sci; SOX13/TRIM11/YAP axis promotes the proliferation, migration and chemoresistance of anaplastic thyroid cancer.; Authors - Jianing Tang
human	WB,IF,CoIP	Neoplasia; TRIM11 promotes breast cancer cell proliferation by stabilizing estrogen receptor α.; Authors - Jianing Tang ; KD Validated