TREM1 Polyklonaler Antikörper

TREM1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 11791-1-AP

Synonyme

TREM 1, CD354

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Galerie der Validierungsdaten

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  WB analysis of human peripheral blood leukocyte using 11791-1-AP

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:450 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11791-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11791-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11791-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 11791-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11791-1-AP (TREM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11791-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11791-1-AP (TREM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11791-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11791-1-AP (TREM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human peripheral blood leukocyte, humanes Lebergewebe, humanes Milzgewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11791-1-AP bindet in WB, IHC, IF, CoIP, ELISA TREM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TREM1 fusion protein Ag2418
• Vollständiger Name: triggering receptor expressed on myeloid cells 1
• Berechnetes Molekulargewicht: 234 aa, 26 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC017773
• Gene symbol: TREM1
• Gene ID (NCBI): 54210
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Triggering receptor expressed on myeloid cells 1 (TREM1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers.

Publikationen

Species	Application	Title
mouse	WB	Theranostics; A COX-2/sEH dual inhibitor PTUPB alleviates lipopolysaccharide-induced acute lung injury in mice by inhibiting NLRP3 inflammasome activation.; Authors - Hui-Hui Yang
mouse	WB	Int Immunopharmacol; Activation of NLRP3 inflammasome up-regulates TREM-1 expression in murine macrophages via HMGB1 and IL-18.; Authors - Wen-Jing Zhong
mouse	IF	Acta Trop; Malaria-derived exosomes exacerbate liver injury during blood stage of Plasmodium berghei infection; Authors - Xin Zhang
human	WB,IHC	J Inflamm Res; Overexpression of TREM1 is Associated with the Immune-Suppressive Microenvironment and Unfavorable Prognosis in Pan-Cancer; Authors - Xinwei Zhou
human	IF	Neurol Neuroimmunol Neuroinflamm; Jo-1 Antibodies From Myositis Induce Complement-Dependent Cytotoxicity and TREM-1 Upregulation in Muscle Endothelial Cells; Authors - Masaya Honda
mouse	IF,WB	Behav Brain Res; Inhibition of TREM-1 Ameliorates Lipopolysaccharide-induced Depressive-like Behaviors by Alleviating Neuroinflammation in the PFC via PI3K/Akt Signaling Pathway; Authors - Anhui Fu