transgelin/SM22-specific Polyklonaler Antikörper

transgelin/SM22-specific Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15502-1-AP

Synonyme

SM22, SM22 alpha, SM22a, SM22alpha, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1

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Galerie der Validierungsdaten

• 

  WB analysis of mouse colon using 15502-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human kidney using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:500 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:600
• Immunhistochemie (IHC): IHC : 1:100-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

15502-1-AP bindet in WB, IHC, ELISA transgelin/SM22-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: transgelin
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: NM_003186
• Gene symbol: TAGLN
• Gene ID (NCBI): 6876
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody is specific to transgelin 1. It does not cross-react with transgelin 2 or 3.

Publikationen

Species	Application	Title
mouse	WB	Physiol Genomics; Inflammation and vascular smooth muscle cell dedifferentiation following carotid artery ligation; Authors - B. Paul Herring
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He
human	WB	Front Genet; Bioinformatics Analysis Reveals MicroRNA-193a-3p Regulates ACTG2 to Control Phenotype Switch in Human Vascular Smooth Muscle Cells.; Authors - Weitie Wang