TRAK2 Polyklonaler Antikörper

TRAK2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13770-1-AP

Synonyme

Trafficking kinesin-binding protein 2, KIAA0549, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein, ALS2CR3

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Galerie der Validierungsdaten

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  WB analysis of human liver using 13770-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13770-1-AP (TRAK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13770-1-AP

  IP result of anti-TRAK2 (IP:13770-1-AP, 4ug; Detection:13770-1-AP 1:500) with HepG2 cells lysate 4000ug.

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  IHC staining of human liver cancer using 13770-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13770-1-AP (TRAK2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 13770-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13770-1-AP (TRAK2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 13770-1-AP

  Immunofluorescent analysis of Hela cells, using TRAK2 antibody 13770-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 13770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAK2 antibody (13770-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13770-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13770-1-AP (TRAK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

13770-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TRAK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAK2 fusion protein Ag4753
• Vollständiger Name: trafficking protein, kinesin binding 2
• Berechnetes Molekulargewicht: 914 aa, 101 kDa
• Beobachtetes Molekulargewicht: 101-106 kDa
• GenBank-Zugangsnummer: BC040668
• Gene symbol: TRAK2
• Gene ID (NCBI): 66008
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Trafficking kinesin-binding protein 2 (TRAK2) is a member of the TRAK family of kinesin adaptor proteins. In mammals, there are two members of the TRAK family, TRAK1 and TRAK2 (PMID: 25653102). TRAK1 and TRAK2 play roles in lysosomal and mitochondrial trafficking in cells. They function as kinesin adaptors linking kinesin heavy chain (KHC) to mitochondria (PMID: 28364549). TRAK2 has been found to act as a trafficking factor for the K+ channel Kir2.1 and γ-Aminobutyric acid type A (GABAA) receptor (PMID: 16895905; 24122114).

Publikationen

Species	Application	Title
human	WB	FASEB J; Adaptation of mitochondrial network dynamics and velocity of mitochondrial movement to chronic stress present in fibroblasts derived from patients with sporadic form of Alzheimer's disease.; Authors - Karolina Drabik
rat	WB	J Cell Sci; Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19.; Authors - Katarzyna Majstrowicz
mouse	WB	Exp Cell Res; Antagomirs targeting miR-142-5p attenuate pilocarpine-induced status epilepticus in mice.; Authors - Haifeng Zhang
rat	WB	Bioeng Transl Med; Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36; Authors - Zhijie Zhu