TRAF5 Polyklonaler Antikörper

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• KD/KO Validated

TRAF5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12868-1-AP

Synonyme

RING finger protein 84, RNF84, TRAF5

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 12868-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12868-1-AP (TRAF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12868-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12868-1-AP (TRAF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12868-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12868-1-AP (TRAF5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12868-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12868-1-AP (TRAF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, Maus-Pankreasgewebe, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

12868-1-AP bindet in WB, IHC, ELISA TRAF5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAF5 fusion protein Ag3519
• Vollständiger Name: TNF receptor-associated factor 5
• Berechnetes Molekulargewicht: 557 aa, 64 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC029600
• Gene symbol: TRAF5
• Gene ID (NCBI): 7188
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du
human	WB	J Cell Mol Med; Down-regulated HDAC3 elevates microRNA-495-3p to restrain epithelial-mesenchymal transition and oncogenicity of melanoma cells via reducing TRAF5.; Authors - Yanbo Ma ; KD Validated
mouse	WB,IHC	Eur J Pharmacol; TRAF5 protects against myocardial ischemia reperfusion injury via AKT signaling.; Authors - Weipan Xu ; KO Validated
human	WB	Med Oncol; DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway.; Authors - Wei Huang
human,human	WB	Transl Cancer Res; Effects of antisense lncRNA PCBP1-AS1 on biological behaviors of vulvar squamous carcinoma cells by regulating TRAF5 and NF-κB expression; Authors - Hongyu Wang