TRAF1 Polyklonaler Antikörper

TRAF1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11543-1-AP

Synonyme

EBI6, TRAF1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11543-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11543-1-AP (TRAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11543-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11543-1-AP (TRAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11543-1-AP bindet in WB, ELISA TRAF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAF1 fusion protein Ag2131
• Vollständiger Name: TNF receptor-associated factor 1
• Berechnetes Molekulargewicht: 416 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46-50 kDa
• GenBank-Zugangsnummer: BC024145
• Gene symbol: TRAF1
• Gene ID (NCBI): 7185
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAF1 is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. TRAF1 and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. TRAF1 is observed at 45-50 kDa in western blotting, and the additional bands of lower molecular weight (~30 kDa) may represent TRAF1 cleavage fragments, similar to the results obtained by the article (PMID:11098060).

Publikationen

Species	Application	Title
mouse	WB	Eur J Nutr; Trans-10-hydroxy-2-decenoic acid protects against LPS-induced neuroinflammation through FOXO1-mediated activation of autophagy.; Authors - Mengmeng You