TPT1 Polyklonaler Antikörper

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• KD/KO Validated

TPT1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10824-1-AP

Synonyme

Translationally-controlled tumor protein, TCTP, p23, HRF, Histamine-releasing factor

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 10824-1-AP

  WB result of TPT1 antibody (10824-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells.

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  WB analysis of mouse placenta using 10824-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10824-1-AP (TPT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10824-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10824-1-AP (TPT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 10824-1-AP

  IP result of anti-TPT1 (IP:10824-1-AP, 4ug; Detection:10824-1-AP 1:500) with human placenta tissue lysate 1520ug.

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  IHC staining of human colon cancer using 10824-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10824-1-AP (TPT1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10824-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10824-1-AP (TPT1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10824-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TPT1 antibody (10824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maus-Plazenta-Gewebe, MCF-7-Zellen
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

10824-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TPT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPT1 fusion protein Ag1268
• Vollständiger Name: tumor protein, translationally-controlled 1
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC003352
• Gene symbol: TPT1
• Gene ID (NCBI): 7178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22-25 kDa TPT1 in lysates. An additional band around 50 kDa can also be observed occasionally, possibly representing the dimer form of TPT1.

Publikationen

Species	Application	Title
human	WB	Pharmacol Res; Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect.; Authors - Dongrong Zhu
human,mouse	WB,IHC,IF	Int J Biol Sci; Ailanthus Altissima-derived Ailanthone enhances Gastric Cancer Cell Apoptosis by Inducing the Repression of Base Excision Repair by Downregulating p23 Expression.; Authors - Chun-Ming Wang
human	WB	Chem Biol Interact; AMG900 as novel inhibitor of the translationally controlled tumor protein.; Authors - Nicolas Fischer
human	IP	Invest New Drugs; A novel ligand of the translationally controlled tumor protein (TCTP) identified by virtual drug screening for cancer differentiation therapy.; Authors - Nicolas Fischer
rat	WB,IHC	BMC Cancer; Identification of proteins involved in neural progenitor cell targeting of gliomas.; Authors - Staflin Karin K
human	WB	Cancer Sci; LncRNA TPT1-AS1 promotes tumorigenesis and metastasis in epithelial ovarian cancer by inducing TPT1 expression.; Authors - Weimin Wu ; KD Validated