TPSAB1 Polyklonaler Antikörper

TPSAB1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13343-1-AP

Synonyme

Tryptase 1, TPSB1, TPS2, TPS1, MCP7

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Galerie der Validierungsdaten

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  WB analysis of A431 using 13343-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 13343-1-AP

  IP result of anti-TPSAB1 (IP:13343-1-AP, 4ug; Detection:13343-1-AP 1:800) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human tonsillitis using 13343-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13343-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendix using 13343-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendix using 13343-1-AP (TPSAB1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human tonsillitis using 13343-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human tonsillitis using 13343-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IF Staining of mouse skin using 13343-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TPSAB1 antibody (13343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 13343-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13343-1-AP (TPSAB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of U-937 using 13343-1-AP

  1X10^6 U-937 cells were intracellularly stained with 0.2 ug Anti-Human TPSAB1 (13343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, K-562-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Appendix-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushautgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

13343-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA TPSAB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPSAB1 fusion protein Ag4167
• Vollständiger Name: tryptase alpha/beta 1
• Berechnetes Molekulargewicht: 275 aa, 31 kDa
• Beobachtetes Molekulargewicht: 32-38 kDa
• GenBank-Zugangsnummer: BC028059
• Gene symbol: TPSAB1
• Gene ID (NCBI): 7177
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Clin Transl Med; Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour; Authors - Kaiyuan Ji
rat	IHC	Front Immunol; Palmatine treats urticaria by reducing inflammation and increasing autophagy; Authors - Tian Xiao
human	IHC	IUBMB Life; Bromodomain-containing protein 9 is a prognostic biomarker associated with immune infiltrates and promotes tumor malignancy through activating notch signaling pathway in negative HIF-2α clear cell renal cell carcinoma.; Authors - Weijuan Lou
human	WB	BMC Cancer; The release of tryptase from mast cells promote tumor cell metastasis via exosomes.; Authors - Hui Xiao
mouse	WB,IF	Int J Mol Sci; An Amide Alkaloid Isolated from Ephedra sinica Ameliorates OVA-Induced Allergic Asthma by Inhibiting Mast Cell Activation and Dendritic Cell Maturation; Authors - Jufang Jia
mouse	IF	Ann Transl Med; Identification of hub genes and infiltrating immune cells in skeletal muscle in obesity; Authors - Shuoshuo Jin