TPM4 Polyklonaler Antikörper

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TPM4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 13741-1-AP

Synonyme

TM30p1, TPM4, tropomyosin 4, Tropomyosin alpha 4 chain

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Galerie der Validierungsdaten

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  WB analysis using 13741-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13741-1-AP (TPM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13741-1-AP

  WB result of TPM4 antibody (13741-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM4 transfected HeLa cells.

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  IHC staining of human endometrial cancer using 13741-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13741-1-AP (TPM4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 13741-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13741-1-AP (TPM4 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, HeLa-Zellen, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

13741-1-AP bindet in WB, IHC, ELISA TPM4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPM4 fusion protein Ag4679
• Vollständiger Name: tropomyosin 4
• Berechnetes Molekulargewicht: 248 aa, 29 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC037576
• Gene symbol: TPM4
• Gene ID (NCBI): 7171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPM4, also named as TM30p1, Tropomyosin 4, Tropomyosin alpha 4 chain. This gene is one gene type of tropomyosin isoforms and encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is a major regulatory protein of cytoskeleton and is localized along actin filaments modulating the interaction of actin and myosin (PubMed: 26315888, PubMed: 32638329). In addition, TPM4 has been reported as an oncoprotein in several malignancies (PubMed: 37459535). The calculated molecular weight of TPM4 is usually approximately 28 kDa. It maybe have two isoforms: the short 30-kDa isoform (TPM4.2) and the long 38-kDa isoform (TPM4.1) (PubMed: 28134622).

Publikationen

Species	Application	Title
mouse	WB	J Proteomics; Network of brain protein level changes in glutaminase deficient fetal mice.; Authors - Bae Narkhyun N
human	WB	Onco Targets Ther; WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.; Authors - Jingjing Dai
human	WB,IHC	Int J Med Sci; The expression and clinical significance of TPM4 in hepatocellular carcinoma.; Authors - Linjing Li
human	WB	mSphere; Ehrlichia chaffeensis TRP75 Interacts with Host Cell Targets Involved in Homeostasis, Cytoskeleton Organization, and Apoptosis Regulation To Promote Infection.; Authors - Tian Luo
human	WB	Osteoporos Int; Differentially expressed proteins identified by TMT proteomics analysis in bone marrow microenvironment of osteoporotic patients.; Authors - Q Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-01-2024)

good for westernblot, decent for immunofluorescence

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCMs