TPM3 Polyklonaler Antikörper

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• KD/KO Validated

TPM3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10737-1-AP

Synonyme

Gamma tropomyosin, hscp30, hTM5, NEM1, OK/SW cl.5, TM 5, TM3, TM30, TM30nm, TPM3, TPMsk3, TRK, tropomyosin 3, Tropomyosin 5, Tropomyosin alpha 3 chain

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 10737-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10737-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10737-1-AP

  WB result of TPM3 antibody (10737-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM3 transfected HepG2 cells.

• 

  WB analysis of HeLa using 10737-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10737-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10737-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 10737-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10737-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10737-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10737-1-AP

  IP result of anti-TPM3 (IP:10737-1-AP, 4ug; Detection:10737-1-AP 1:300) with HeLa cells lysate 2000ug.

• 

  IHC staining of human ovary tumor using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10737-1-AP (TPM3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

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  IHC staining of human ovary using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, Mausherzgewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Eierstockgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

10737-1-AP bindet in WB, IP, IF, IHC, ELISA TPM3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPM3 fusion protein Ag1171
• Vollständiger Name: tropomyosin 3
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC008407
• Gene symbol: TPM3
• Gene ID (NCBI): 7170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tropomyosins are ubiquitous proteins of 35 to 45 kDa associated with the actin filaments of myofibrils and stress fibers. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins.TPM2 subunit constitutes one part of the TPM dimer, and TPM1 or TPM3 constitutes the other part of the TPM dimer (22064019), causing an extra band of ~70kDa.

Publikationen

Species	Application	Title
human	WB	Bone Res; Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma; Authors - Ming Luo
	IP	Nucleic Acids Res; Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.; Authors - Ken Asada
human	WB	Oncogene; MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.; Authors - Agarwal N N
human,mouse	WB	Oncol Res; LncRNA WEE2-AS1 Knockdown Inhibits the Proliferation, Migration and 3 Invasion of Glioma Cells via Regulating miR-29b-2-5p/TPM3 Axis.; Authors - Zhen Jia
	WB	J Proteome Res; Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy; Authors - Yuan Lin
human	IHC	Bioengineered; PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma.; Authors - Kaiming Peng