TPK1 Polyklonaler Antikörper

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• KD/KO Validated

TPK1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10942-1-AP

Synonyme

HTPK1, Placental protein 20, PP20, thiamin pyrophosphokinase 1, Thiamine pyrophosphokinase 1, TPK1

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Galerie der Validierungsdaten

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  WB analysis using 10942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10942-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10942-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10942-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human small intestine using 10942-1-AP

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10942-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 10942-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10942-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10942-1-AP

  IP result of anti-TPK1 (IP:10942-1-AP, 4ug; Detection:10942-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 Antibody) at dilution of 1:50(under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HEK-293-Zellen, humanes Dünndarmgewebe, L02-Zellen, Maus-Dünndarmgewebe, Maushodengewebe, Mausnierengewebe, Ratten-Dünndarmgewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below

Produktinformation

10942-1-AP bindet in WB, IP, IHC, ELISA TPK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPK1 fusion protein Ag1404
• Vollständiger Name: thiamin pyrophosphokinase 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC014552
• Gene symbol: TPK1
• Gene ID (NCBI): 27010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPK1(Thiamin pyrophosphokinase 1) is also named as PP20(placental protein 20) and belongs to the thiamine pyrophosphokinase family. It is a cellular enzyme involved in the regulation of thiamine metabolism and catalyzes the conversion of thiamine, a form of vitamin B1, to thiamine pyrophosphate (TDP, or TPP). There is no difference in TPK1 expression in cultured fibroblasts from normal subjects or from patients with thiamine-responsive megaloblastic anemia(PMID:11342111). It can exsit as a dimer(PMID:21552434). Defects in TPK1 are the cause of thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5).

Publikationen

Species	Application	Title
human	WB	Sci Adv; Dietary thiamine influences l-asparaginase sensitivity in a subset of leukemia cells.; Authors - Rohiverth Guarecuco ; KO Validated
mouse	WB	Proc Natl Acad Sci U S A; OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin.; Authors - Ligong Chen
human	WB	Oncotarget; Identification of vitamin B1 metabolism as a tumor-specific radiosensitizing pathway using a high-throughput colony formation screen.; Authors - Gaganpreet S Tiwana
human	WB	Am J Pathol; Identification of aurora kinase B and Wee1-like protein kinase as downstream targets of (V600E)B-RAF in melanoma.; Authors - Sharma Arati A
rat	WB	Sci Rep; Thiamine metabolism is critical for regulating correlated growth of dendrite arbors and neuronal somata.; Authors - Huimin Liu
human	WB	PLoS One; Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.; Authors - Shuqian Liu