Torsin A Polyklonaler Antikörper

Torsin A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 10296-1-AP

Synonyme

DQ2, Dystonia 1 protein, DYT1, TOR1A, Torsin 1A, Torsin A, Torsin family 1 member A, TorsinA

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10296-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10296-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10296-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10296-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10296-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10296-1-AP (Torsin A antibody) at dilution of 1:100 (under 25x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, Mauslebergewebe, Mausnierengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10296-1-AP bindet in WB, IHC, ELISA Torsin A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Torsin A fusion protein Ag0358
• Vollständiger Name: torsin family 1, member A (torsin A)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 42-50 kDa
• GenBank-Zugangsnummer: BC000674
• Gene symbol: TOR1A
• Gene ID (NCBI): 1861
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOR1A (torsin A), also known as DYT1, is a member of AAA+ family of ATPases that mediate chaperone and other functions involved in conformational modeling of proteins, protection from stress, and targeting of proteins to cellular organelles. TOR1A is a 37 kDa ATP binding protein highly expressed in hippocampus, spinal cord, kidney and liver. TOR1A primarily localizes to the ER while exposure to oxidative stress causes the redistribution to cell surface protrusions. Mutations in gene encoding TOR1A cause the most severe form of hereditary dystonia, termed early onset, generalized dystonia. Interestingly, Lewy bodies of PD patients were intensely immunoreactive for torsinA.

Publikationen

Species	Application	Title
mouse	WB	Neurobiol Dis; Postnatal Foxp2 regulates early psychiatric-like phenotypes and associated molecular alterations in the R6/1 transgenic mouse model of Huntington's disease; Authors - Ened Rodríguez-Urgellés