Tom22 Monoklonaler Antikörper

Tom22 Monoklonal Antikörper für FC (Intra), WB

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1C2B11

Kat-Nr. : CL594-66562

Synonyme

1C9 2, hTom22, MST065, MSTP065, TOM22, TOMM22

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Galerie der Validierungsdaten

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  WB analysis using CL594-66562

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-66562 (TOMM22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293 using CL594-66562

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Tom22 (CL594-66562, Clone:1C2B11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66562 bindet in WB, FC (Intra) Tom22 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Tom22 fusion protein Ag1797
• Vollständiger Name: translocase of outer mitochondrial membrane 22 homolog (yeast)
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC009363
• Gene symbol: TOMM22
• Gene ID (NCBI): 56993
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOMM22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C