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TNP1 Polyklonaler Antikörper

TNP1 Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-17178

Synonyme

STP 1, TNP1, TP 1, TP1

Galerie der Validierungsdaten

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®555-conjugated TNP1 Polyclonal antibody (CL555-17178)

    IF Staining of mouse testis using CL555-17178

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®555 TNP1 antibody (CL555-17178) at dilution of 1:100, CoraLite® Plus 488 BOULE antibody (CL488-13720, green), STAR antibody (67130-1-Ig, Clone: 1G6E6, Magenta).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®555-conjugated TNP1 Polyclonal antibody (CL555-17178)

    IF Staining of mouse testis using CL555-17178

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®555 TNP1 antibody (CL555-17178) at dilution of 1:100.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMaushodengewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-17178 bindet in IF TNP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TNP1 fusion protein Ag10890
Vollständiger Name transition protein 1 (during histone to protamine replacement)
Berechnetes Molekulargewicht 55 aa, 7 kDa
Beobachtetes Molekulargewicht 6-10 kDa
GenBank-ZugangsnummerBC029516
Gene symbol TNP1
Gene ID (NCBI) 7141
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transition nuclear proteins (TNP1 and TNP2) are the major nuclear proteins that replace somatic histones during spermatogenesis. TNPs are required for normal chromatin condensation and functional sperm development, spermatogenesis was found to be compromised in both Tnp1 and Tnp2 null mice. TNP1, or TP1, localized in nucleus, is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Recently, TNP-1 was used as a germ cell marker in condensing spermatids.

Protokolle

Produktspezifische Protokolle
IF protocol for CL555 TNP1 antibody CL555-17178Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen