CD134/OX40 Polyklonaler Antikörper

CD134/OX40 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20006-1-AP

Synonyme

ACT35, ACT35 antigen, CD134, CD134/OX40, OX40, OX40L receptor, TNFRSF4, TXGP1L

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 20006-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20006-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 20006-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  FC experiment of human PBMCs using 20006-1-AP

  1X10^6 day 3 PHA treated human PBMCs were surface stained with 0.2 ug Anti-Human CD134/OX40 (20006-1-AP) and CoraLite®750-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or unstained (blue). Unstimulated human PBMCs were surface stained with 0.2ug Anti-Human CD134/OX40 (20006-1-AP) and CoraLite®750-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (black dashed). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, L02-Zellen, U-937-Zellen
• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

20006-1-AP bindet in WB, FC, ELISA CD134/OX40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 4
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: NM_003327
• Gene symbol: TNFRSF4
• Gene ID (NCBI): 7293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5.

Publikationen

Species	Application	Title
human	WB	Front Immunol; A Novel Immune-Related Gene Signature to Identify the Tumor Microenvironment and Prognose Disease Among Patients With Oral Squamous Cell Carcinoma Patients Using ssGSEA: A Bioinformatics and Biological Validation Study; Authors - Yun Chen
human	WB	Dis Markers; Development of a Costimulatory Molecule Signature to Predict Prognosis, Immune Landscape, and Response to Immune Therapy for Hepatocellular Carcinoma; Authors - Yongjie Zhou

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