TNF-alpha Polyklonaler Antikörper

TNF-alpha Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 26162-1-AP

Synonyme

DIF, Tnf, TNF alpha, Tnfa, TNFalpha, TNF-alpha, Tnfsf1a, TNFSF2, TNFα, TNF-α, tumor necrosis factor

---

• 

  WB analysis using 26162-1-AP

  Untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26162-1-AP (Tnf alpha antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

26162-1-AP bindet in WB, IHC, IF, ELISA TNF-alpha und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNF-alpha fusion protein Ag24089
• Vollständiger Name: tumor necrosis factor
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: NM-013693
• Gene symbol: TNF-alpha
• Gene ID (NCBI): 21926
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Mouse and human TNF-alpha share 79% amino acid sequence identity. Unlike human TNF-alpha, the mouse form is glycosylated. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles.

Publikationen

Species	Application	Title
mouse	WB	Cell Signal; ISG15 increases the apoptosis of β cells in type 1 diabetes; Authors - Jie Liu
mouse	WB	Biochem Biophys Res Commun; Uric acid-lowering effect of harpagoside and its protective effect against hyperuricemia-induced renal injury in mice; Authors - Qiong Fu
mouse	WB	J Control Release; Microenvironment-responsive nano-bioconjugated vesicles for the multi-pronged treatment of liver fibrosis; Authors - Jie-Hua Xing
rat	IHC	ACS Appl Mater Interfaces; Evaluation of the Antibacterial, Anti-Inflammatory, And Bone-Promoting Capacity of UiO-66 Loaded with Thymol or Carvacrol; Authors - Minghe Zheng
mouse	IHC	Cell Mol Biol Lett; Single-cell view and a novel protective macrophage subset in perivascular adipose tissue in T2DM; Authors - Jiaxuan Li
		Neurobiol Dis; Temporal dynamics of neutrophil functions in multiple sclerosis; Authors - Shishi Shen