TMOD4 Polyklonaler Antikörper

TMOD4 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11753-1-AP

Synonyme

SK TMOD, Skeletal muscle tropomodulin, TMOD4, Tropomodulin 4, tropomodulin 4 (muscle)

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 11753-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11753-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11753-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 11753-1-AP

  IP result of anti-TMOD4 (IP:11753-1-AP, 4ug; Detection:11753-1-AP 1:600) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human gliomas using 11753-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11753-1-AP (TMOD4 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, HeLa-Zellen, humanes Hirngewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

11753-1-AP bindet in WB, IP, IF, IHC, ELISA TMOD4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte, Zebrafisch, Krallenfrosch (Xenopus)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMOD4 fusion protein Ag2349
• Vollständiger Name: tropomodulin 4 (muscle)
• Berechnetes Molekulargewicht: 345 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC017810
• Gene symbol: TMOD4
• Gene ID (NCBI): 29765
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMOD4 is a member of tropomodulin (Tmod) family proteins which are capping proteins binding to the pointed end of actin filaments and preventing both elongation and depolymerization. Four mammalian Tmod genes have been identified to date. Tomd1 is expressed in terminally-differentiated post-mitotic cells, like heart, brain, lens, vasculature, and erythrocytes; Tmod2 expressed in neurons; Tmod3 expressed ubiquitously; Tmod4 expressed only in skeletal muscles. This antibody was raised against the full-length human TMOD4 and may cross-react with other Tmods.

Publikationen

Species	Application	Title
mouse	WB	Elife; Downsizing the molecular spring of the giant protein titin reveals that skeletal muscle titin determines passive stiffness and drives longitudinal hypertrophy.; Authors - Ambjorn Brynnel
Xenopus	WB, IF	J Cell Sci; Leiomodin 3 and Tropomodulin 4 have overlapping functions during skeletal myofibrillogenesis.; Authors - Chinedu U Nworu
zebrafish	IHC	Dis Model Mech; Neb: a zebrafish model of nemaline myopathy due to nebulin mutation.; Authors - Telfer William R WR
zebrafish	IF	Dis Model Mech; Loss of tropomodulin4 in the zebrafish mutant träge causes cytoplasmic rod formation and muscle weakness reminiscent of nemaline myopathy.; Authors - Joachim Berger
rat	WB	J Proteome Res; Proteomic and Phosphoproteomic Analysis of Right Ventricular Hypertrophy in the Pulmonary Hypertension Rat Model; Authors - Ang Luo
rat	IF	Front Physiol; Titin isoform size is not correlated with thin filament length in rat skeletal muscle.; Authors - Marion L Greaser