TMEM165 Polyklonaler Antikörper

TMEM165 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 20485-1-AP

Synonyme

TMEM165, TMPT27, TPARL, transmembrane protein 165, Transmembrane protein PT27, Transmembrane protein TPARL

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 20485-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20485-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20485-1-AP

  IP Result of anti-TMEM165 (IP:20485-1-AP, 4ug; Detection:20485-1-AP 1:1000) with HeLa cells lysate 3440ug.

• 

  IF Staining of HepG2 using 20485-1-AP

  Immunofluorescent analysis of HepG2 cells using 20485-1-AP (TMEM165 antibody) at dilution of 1:100 and Rhodamine-labeled goat anti-rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20485-1-AP bindet in WB, IP, IHC, IF, ELISA TMEM165 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM165 fusion protein Ag14362
• Vollständiger Name: transmembrane protein 165
• Berechnetes Molekulargewicht: 324 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC003545
• Gene symbol: TMEM165
• Gene ID (NCBI): 55858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT), a member of methyltransferase superfamily, is an enzyme involved in the normal metabolic inactivation of thiopurine drugs such as azathioprine, 6-mercaptopurine and 6-thioguanine. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Defects in the TPMT gene leads to decreased methylation and decreased inactivation of 6MP leading to enhanced bone marrow toxicity which may cause myelosuppression, anemia, bleeding tendency, leukopenia and infection.

Publikationen

Species	Application	Title
mouse	WB	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
human	IF, WB	J Cell Biol; Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.; Authors - Thaddaeus Mutugi Nthiga
	WB	J Biol Chem; The human Golgi protein TMEM165 transports calcium and manganese in yeast and bacterial cells.; Authors - Jiri Stribny
human	WB	Oncol Rep; TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity.; Authors - Jee-San Lee
mouse	WB	Biochem Biophys Res Commun; The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca2+ATPase (SPCA1).; Authors - Timothy A Reinhardt
human	IHC	J Hepatocell Carcinoma; Identification of a Novel Prognostic Signature Based on N-Linked Glycosylation and Its Correlation with Immunotherapy Response in Hepatocellular Carcinoma; Authors - Shusheng Lin