TM7SF2 Polyklonaler Antikörper

TM7SF2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12033-1-AP

Synonyme

C14SR, C-14 sterol reductase, C 14 sterol reductase, Another new gene 1 protein, 3-beta-hydroxysterol Delta

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Galerie der Validierungsdaten

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  WB analysis of human lung using 12033-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12033-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12033-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 12033-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12033-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12033-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12033-1-AP (TM7SF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12033-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12033-1-AP (TM7SF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 12033-1-AP

  Immunofluorescent analysis of PC-3 cells using 12033-1-AP (TM7SF2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lungengewebe, BxPC-3-Zellen, humanes Herzgewebe, humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12033-1-AP bindet in WB, IHC, IF/ICC, ELISA TM7SF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TM7SF2 fusion protein Ag2637
• Vollständiger Name: transmembrane 7 superfamily member 2
• Berechnetes Molekulargewicht: 418 aa, 46 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC012857
• Gene symbol: TM7SF2
• Gene ID (NCBI): 7108
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	PLoS One; Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of cyp51 from cholesterol synthesis.; Authors - Monika Lewinska