TLE3 Monoklonaler Antikörper

TLE3 Monoklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IP, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

1D11A8

Kat-Nr. : 66083-1-Ig

Synonyme

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66083-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66083-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 66083-1-Ig

  IP Result of anti-TLE3 (IP:66083-1-Ig, 5ug; Detection:66083-1-Ig 1:500) with HEK-293 cells lysate 1480ug.

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  IHC staining of human breast cancer using 66083-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 66083-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 293 cells, HEK-
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66083-1-Ig bindet in WB, IP, IHC,ELISA TLE3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TLE3 fusion protein Ag17906
• Vollständiger Name: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Berechnetes Molekulargewicht: 772 aa, 83 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC015729
• Gene symbol: TLE3
• Gene ID (NCBI): 7090
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )