Transketolase Polyklonaler Antikörper

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• KD/KO Validated

Transketolase Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 11039-1-AP

Synonyme

TKT, TK, EC:2.2.1.1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11039-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11039-1-AP (Transketolase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 11039-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11039-1-AP (Transketolase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11039-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11039-1-AP (Transketolase antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 11039-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11039-1-AP (Transketolase antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11039-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11039-1-AP (Transketolase antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11039-1-AP (Transketolase antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11039-1-AP (Transketolase antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of BxPC-3 using 11039-1-AP

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using Transketolase antibody (11039-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Kolongewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:100-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 19 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Produktinformation

11039-1-AP bindet in WB, IHC, IF/ICC, ELISA Transketolase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Transketolase fusion protein Ag1519
• Vollständiger Name: transketolase
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC009970
• Gene symbol: Transketolase
• Gene ID (NCBI): 7086
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

Publikationen

Species	Application	Title
human		Mol Psychiatry; A cross-species spatiotemporal proteomic analysis identifies UBE3A-dependent signaling pathways and targets.; Authors - Nikhil J Pandya
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
mouse	WB	Proc Natl Acad Sci U S A; OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin.; Authors - Ligong Chen
human	WB	J Hazard Mater; Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.; Authors - Ye Zhu
human	WB	Cancer Lett; Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.; Authors - Xintian Chen
human	WB,IHC	Eur Respir J; Malignant pleural effusion cells show aberrant glucose metabolism gene expression.; Authors - Lin C-C CC ; KD Validated