TINAGL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TINAGL1 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12077-1-AP

Synonyme

PP6614, Oxidized LDL-responsive gene 2 protein, OLRG-2, LCN7, Glucocorticoid-inducible protein 5

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Galerie der Validierungsdaten

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  WB analysis using 12077-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12077-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 12077-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12077-1-AP

  IP result of anti-TINAGL1 (IP:12077-1-AP, 4ug; Detection:12077-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human liver cancer using 12077-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12077-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 12077-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TINAGL1 antibody (12077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, humanes Nebennierengewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1500
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

12077-1-AP bindet in WB, IHC, IF-P, IP, ELISA TINAGL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TINAGL1 fusion protein Ag2721
• Vollständiger Name: tubulointerstitial nephritis antigen-like 1
• Berechnetes Molekulargewicht: 467 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC009048
• Gene symbol: TINAGL1
• Gene ID (NCBI): 64129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tubulointerstitial nephritis antigen-like 1 (Tinagl1), a secreted extracellular protein, was initially identified as a putative component of the ECM. Tinagl1 is most commonly associated with embryonic development, including the heart and adrenal glands. Low levels of Tinagl1 expression can be dire for craniofacial development and for female fertility.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	FASEB J; Down-regulated TINAGL1 in fibroblasts impairs wound healing in diabetes.; Authors - Wen-Qing Tian
human,mouse	WB	Biochim Biophys Acta Mol Cell Res; Functional analysis reveals that Tinagl1 is required for normal muscle development in mice through the activation of ERK signaling.; Authors - Yoriko Sato ; KD Validated
human	WB,IP	J Transl Med; Tubulointerstitial nephritis antigen-like 1 from cancer-associated fibroblasts contribute to the progression of diffuse-type gastric cancers through the interaction with integrin β1; Authors - Dagyeong Lee
human	WB	Cancer Cell; Tinagl1 Suppresses Triple-Negative Breast Cancer Progression and Metastasis by Simultaneously Inhibiting Integrin/FAK and EGFR Signaling.; Authors - Minhong Shen
human	WB	Cancer Manag Res; TINAGL1 promotes hepatocellular carcinogenesis through the activation of TGF-β signaling-medicated VEGF expression.; Authors - Lu Sun ; KD Validated