TIMP-4 Polyklonaler Antikörper

TIMP-4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 12326-1-AP

Synonyme

TIMP4, TIMP 4, Metalloproteinase inhibitor 4

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Galerie der Validierungsdaten

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  WB analysis of fetal human brain using 12326-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 12326-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12326-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12326-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12326-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 12326-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12326-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12326-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 12326-1-AP

  Immunofluorescent analysis of MCF-7 cells using 12326-1-AP (TIMP-4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, BxPC-3-Zellen, HT-1080.Zellen, humanes Herzgewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 1 publications below

Produktinformation

12326-1-AP bindet in WB, IHC, IF/ICC, ELISA TIMP-4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIMP-4 fusion protein Ag2927
• Vollständiger Name: TIMP metallopeptidase inhibitor 4
• Berechnetes Molekulargewicht: 224 aa, 26 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC010553
• Gene symbol: TIMP4
• Gene ID (NCBI): 7079
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
mouse	WB,IF	Viruses; Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model.; Authors - Wangquan Ji
human	WB	J Biochem Mol Toxicol; Pacenta polypeptide injection alleviates the fibrosis and inflammation in cigarette smoke extracts-induced BEAS-2B cells by modulating MMP-9/TIMP-1 signaling; Authors - Wei Xu
human	WB	Biochem Biophys Res Commun; Molecularly targeted anti-cancer drugs inhibit the invasion and metastasis of hepatocellular carcinoma by regulating the expression of MMP and TIMP gene families.; Authors - Xiao-Xiao He
mouse	WB	PLoS One; Fructose-1,6-bisphosphate prevents pulmonary fibrosis by regulating extracellular matrix deposition and inducing phenotype reversal of lung myofibroblasts.; Authors - Henrique Bregolin Dias
human	WB	Biomed Res Int; Apatinib Inhibits the Invasion and Metastasis of Liver Cancer Cells by Downregulating MMP-Related Proteins via Regulation of the NF-κB Signaling Pathway.; Authors - Xiaoxiao He