TIMELESS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TIMELESS Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14421-1-AP

Synonyme

hTIM, Protein timeless homolog, TIM, TIM1, TIMELESS, timeless homolog (Drosophila), TIMELESS1

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Galerie der Validierungsdaten

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  WB analysis using 14421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14421-1-AP (TIMELESS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14421-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14421-1-AP (TIMELESS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14421-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14421-1-AP (TIMELESS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14421-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Eierstockgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

14421-1-AP bindet in WB, IHC, IF, ELISA TIMELESS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIMELESS fusion protein Ag5767
• Vollständiger Name: timeless homolog (Drosophila)
• Berechnetes Molekulargewicht: 139 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC050557
• Gene symbol: TIMELESS
• Gene ID (NCBI): 8914
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Lett; TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.; Authors - Sai-Lan Liu ; KD Validated
human	WB	Cell Death Dis; MEX3A promotes the malignant progression of ovarian cancer by regulating intron retention in TIMELESS.; Authors - Fangfang Li ; KD Validated
human	WB	Cell Prolif; Circadian clock genes promote glioma progression by affecting tumour immune infiltration and tumour cell proliferation.; Authors - Zeyu Wang
human	WB,IHC	Gut Microbes; Exosomal-miR-129-2-3p derived from Fusobacterium nucleatum-infected intestinal epithelial cells promotes experimental colitis through regulating TIMELESS-mediated cellular senescence pathway; Authors - Shuchun Wei ; KD Validated