TGM3 Polyklonaler Antikörper

TGM3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21406-1-AP

Synonyme

TG(E), TGase 3, TGase E, TGE, TGM3, Transglutaminase 3, Transglutaminase E

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Galerie der Validierungsdaten

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  WB analysis using 21406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21406-1-AP (TGM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SMMC-7721-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21406-1-AP bindet in WB, ELISA TGM3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGM3 fusion protein Ag16131
• Vollständiger Name: transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Berechnetes Molekulargewicht: 693 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa; 30-35 kDa; 44-50 kDa
• GenBank-Zugangsnummer: BC109076
• Gene symbol: TGM3
• Gene ID (NCBI): 7053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGM3 (Transglutaminase 3) is a Protein Coding gene. Diseases associated with TGM3 include Uncombable Hair Syndrome 2 and Uncombable Hair Syndrome 1. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. TG3 is expressed as an inactive 77 kDa zymogen which must be activated by limited proteolytic processing into two fragments (44 kDa and 30 kDa) of which the larger, N-terminal fragment carries the catalytic activity (PMID: 35328331).