TGM2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TGM2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (1)

Kat-Nr. : 15100-1-AP

Synonyme

G ALPHA h, GNAH, TG(C), TG2, TGase 2, TGase C, TGase H, TGC, TGM2, Tissue transglutaminase, Transglutaminase 2, Transglutaminase C, Transglutaminase H

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Galerie der Validierungsdaten

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  WB analysis using 15100-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15100-1-AP (TGM2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15100-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15100-1-AP (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15100-1-AP

  WB result of TGM2 antibody (15100-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGM2 transfected HepG2 cells.

• 

  WB analysis of HUVEC using 15100-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 15100-1-AP (TGM2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15100-1-AP

  IP Result of anti-TGM2 (IP:15100-1-AP, 4ug; Detection:15100-1-AP 1:400) with HeLa cells lysate 1200ug.

• 

  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen, A549-Zellen, BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen, HUVEC-Zellen, K-562-Zellen, SGC-7901-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 17 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Produktinformation

15100-1-AP bindet in WB, IP, IHC, IF, ELISA TGM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGM2 fusion protein Ag7439
• Vollständiger Name: transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 77-80 kDa
• GenBank-Zugangsnummer: BC003551
• Gene symbol: TGM2
• Gene ID (NCBI): 7052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Nature; Autoimmune amelogenesis imperfecta in patients with APS-1 and coeliac disease; Authors - Yael Gruper
human	WB,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	WB,IF	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	WB,IHC	Cell Oncol (Dordr); Transglutaminases are oncogenic biomarkers in human cancers and therapeutic targeting of TGM2 blocks chemoresistance and macrophage infiltration in pancreatic cancer; Authors - Shan Zhang ; KD Validated
human	WB	Int J Mol Sci; TGFβ-1 Induced Cross-Linking of the Extracellular Matrix of Primary Human Dermal Fibroblasts.; Authors - Mariya E Semkova

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jon (Verified Customer) (02-15-2022)

DILuted as suggested in %5 milk TBST blocking buffer. We have had problems with other TGM2 antibodies from other companies, but this one seemed no work better and yield a better signal. However, the apparent molecular weight of the TGM2 band appeared to be at a much lower MW than it is supposed to.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: EndoCßH1 human pancreatic beta cell line

TGM2 Antibody Western Blot validation (1:1000 dilution) in EndoCßH1 human pancreatic beta cell line (Cat no:15100-1-AP)