TGM1 Polyklonaler Antikörper

TGM1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

30

Kat-Nr. : 12912-3-AP

Synonyme

Epidermal TGase, ICR2, KTG, LI, LI1, TG(K), TGASE, TGase 1, TGase K, TGK, TGM1, Transglutaminase 1, Transglutaminase K

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Galerie der Validierungsdaten

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  WB analysis using 12912-3-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12912-3-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12912-3-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12912-3-AP

  IP result of anti-TGM1 (IP:12912-3-AP, 4ug; Detection:12912-3-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human cervix using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human saliva, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Hautgewebe, humanes Zervixgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 10 publications below
• ELISA: See 1 publications below

Produktinformation

12912-3-AP bindet in WB, IHC, IP, ELISA, IF TGM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGM1 fusion protein Ag3905
• Vollständiger Name: transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
• Berechnetes Molekulargewicht: 817 aa, 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC034699
• Gene symbol: TGM1
• Gene ID (NCBI): 7051
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transglutaminase 1 is an epidermal enzyme that is expressed during the terminal differentiation of keratinized squamous epithelium to form cornified cell envelopes in differentiated keratinocytes. Mutation of TGM1 has been linked to various epidermal diseases. This antibody recognizes the endogenous 90 kDa TGM1 proteins.

Publikationen

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	J Invest Dermatol; Investigation of Omega-3 Polyunsaturated Fatty Acid Biological Activity in a Tissue-Engineered Skin Model Involving Psoriatic Cells.; Authors - Mélissa Simard
human	WB	J Invest Dermatol; Cathelicidin LL-37 Activates Human Keratinocyte Autophagy through the P2X₇, Mechanistic Target of Rapamycin, and MAPK Pathways; Authors - Risa Ikutama
human	IF	Int J Mol Sci; Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model.; Authors - Andréa Tremblay
mouse	WB,ELISA	Cells; Increased Osteoclastogenesis in Absence of TG2 Is Reversed by Transglutaminase Inhibition-Evidence for the Role for TG1 in Osteoclast Formation; Authors - Sahar Ebrahimi Samani
human	WB	Front Endocrinol (Lausanne); c-Myc Upregulated by High Glucose Inhibits HaCaT Differentiation by S100A6 Transcriptional Activation.; Authors - Jie Zhang