HIC5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HIC5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10565-1-AP

Synonyme

Transforming growth factor beta-1-induced transcript 1 protein, TGFB1I1, Hydrogen peroxide-inducible clone 5 protein, Hic-5, ARA55

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Galerie der Validierungsdaten

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  WB analysis using 10565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10565-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 10565-1-AP

  IP result of anti-HIC5 (IP:10565-1-AP, 4ug; Detection:10565-1-AP 1:500) with A431 cells lysate 1520ug.

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  IHC staining of human spleen using 10565-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10565-1-AP (HIC5 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 10565-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10565-1-AP (HIC5 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 10565-1-AP

  Immunofluorescent analysis of A431 cells using 10565-1-AP (HIC5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 10565-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIC5 antibody (10565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, Maus-Kolongewebe, Ratten-Kolongewebe
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

10565-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HIC5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIC5 fusion protein Ag0868
• Vollständiger Name: transforming growth factor beta 1 induced transcript 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC001830
• Gene symbol: HIC5
• Gene ID (NCBI): 7041
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse,rat	IF,WB,IHC	Acta Pharmacol Sin; GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy; Authors - Ji-Chao Wu
human	WB,IF,IHC	Aging (Albany NY); Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.; Authors - Liansheng Sha ; KD Validated
human	WB	Eur J Pharmacol; Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells; Authors - Meifang Zheng
human	WB	J Proteome Res; Quantitative proteomics implicates Rictor/mTORC2 in cell adhesion.; Authors - Hao Wang
human	IHC,IF	Cell Death Dis; HIC-5 in cancer-associated fibroblasts contributes to esophageal squamous cell carcinoma progression.; Authors - Xuanling Du
human	IHC	Med Sci Monit; Bioinformatics Profiling and Experimental Validation of 4 Differentially-Expressed LIM Genes in the Course of Colorectal-Adenoma-Carcinoma; Authors - Ying He