TGF beta 1 Polyklonaler Antikörper

TGF beta 1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26155-1-AP

Synonyme

TGF beta1, Tgfb, Tgfb 1, Tgfb1, TGF-beta 1, TGFbeta1, TGF-beta1, TGF-β, TGFβ1, TGF-β1

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Galerie der Validierungsdaten

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  WB analysis using 26155-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 26155-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26155-1-AP

  NIH3T3 cells were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L-929-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26155-1-AP bindet in WB, ELISA TGF beta 1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGF beta 1 fusion protein Ag24078
• Vollständiger Name: transforming growth factor, beta 1
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: NM-011577
• Gene symbol: Tgfb1
• Gene ID (NCBI): 21803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGF beta 1 is a potent cytokine capable of regulating multiple cellular processes including cell proliferation, differentiation, wound healing, and immune response. TGF beta 1 is identified in various immune cells and its activity varies in different cell types or cells at different developmental stages1. TGF beta 1 functions by binding to the TGFβ1 receptors, which subsequently activate the canonical SMAD pathway or non-canonical signaling cascades, such as the mitogen-activated protein kinase (MAPK) pathway, phosphatidylinositol-3 kinase and AKT (PI3K-AKT), and Rho family of GTPases (Rho GTPase). What is special about TGF beta 1 is its context-dependent nature that is particularly true for tumors. Full-length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).