TFEC Polyklonaler Antikörper

TFEC Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 13547-1-AP

Synonyme

bHLHe34, hTFEC L, TCFEC, TFE C, TFEC, TFECL, transcription factor EC, Transcription factor EC like

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 13547-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13547-1-AP (TFEC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 13547-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13547-1-AP (TFEC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13547-1-AP

  IP result of anti-TFEC (IP:13547-1-AP, 4ug; Detection:13547-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, COLO 320-Zellen
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13547-1-AP bindet in WB, IP, ELISA TFEC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFEC fusion protein Ag4061
• Vollständiger Name: transcription factor EC
• Berechnetes Molekulargewicht: 347 aa, 39 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa, 65 kDa
• GenBank-Zugangsnummer: BC029891
• Gene symbol: TFEC
• Gene ID (NCBI): 22797
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFEC shares a bHLH/LZ structure with TFE3 and a closely related protein microphthalmia-associated transcription factor (MITF), which is critically involved in melanocyte differentiation. The expression of TFEC is mainly in fibroblasts, myoblasts, chondrosarcoma cells and myeloma cells. 50kDa TFEC isoforms are produced by alternative splicing (PMID: 29303964, PMID: 11467950).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Weihao (Verified Customer) (02-03-2022)

Works great for mouse BMDM and human MDM, strong signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500