TFEB Monoklonaler Antikörper
TFEB Monoklonal Antikörper für WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human
Anwendung
WB, ELISA
Konjugation
Unkonjugiert
CloneNo.
1C2D9
Kat-Nr. : 68632-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | SK-BR-3 cells, Daudi-Zellen, HepG2-Zellen, Raji-Zellen, Ramos-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
68632-1-Ig bindet in WB, ELISA TFEB und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | TFEB fusion protein Ag24850 |
Vollständiger Name | transcription factor EB |
Berechnetes Molekulargewicht | 476 aa, 53 kDa |
Beobachtetes Molekulargewicht | 53, 65-70 kDa |
GenBank-Zugangsnummer | BC032448 |
Gene symbol | TFEB |
Gene ID (NCBI) | 7942 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).
Protokolle
Produktspezifische Protokolle | |
---|---|
WB protocol for TFEB antibody 68632-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |