TFDP2 Polyklonaler Antikörper

TFDP2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11500-1-AP

Synonyme

Transcription factor Dp-2, DP2, Dp 2

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 11500-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11500-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11500-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 11500-1-AP

  IP result of anti-TFDP2 (IP:11500-1-AP, 4ug; Detection:11500-1-AP 1:400) with SH-SY5Y cells lysate 1600ug.

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  IP experiment of SH-SY5Y using 11500-1-AP

  IP result of anti-TFDP2 (IP:11500-1-AP, 4ug; Detection:11500-1-AP 1:600) with SH-SY5Y cells lysate 1360 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Hirngewebe
• Erfolgreiche IP: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11500-1-AP bindet in WB, IP, ELISA TFDP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFDP2 fusion protein Ag2054
• Vollständiger Name: transcription factor Dp-2 (E2F dimerization partner 2)
• Berechnetes Molekulargewicht: 446 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC021113
• Gene symbol: TFDP2
• Gene ID (NCBI): 7029
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFDP2, also known as E2FDP2, is a coactivator of E2F factors and involves in EGF or IFN-gamma-mediated effects by enhancing or reducing active heterodimeric complexes. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis [PMID:16360038]. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication [PMID:16721044].

Publikationen

Species	Application	Title
mouse	WB	Mol Syst Biol; The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.; Authors - Caron Etienne E