TERF1 Polyklonaler Antikörper

TERF1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11899-1-AP

Synonyme

hTRF1 AS, NIMA interacting protein 2, PIN2, t TRF1, Telomeric protein Pin2/TRF1, TERF1, TRBF1, TRF, TRF1, TTAGGG repeat binding factor 1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11899-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11899-1-AP (TERF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11899-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11899-1-AP (TERF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11899-1-AP

  IP result of anti-TERF1 (IP:11899-1-AP, 4ug; Detection:11899-1-AP 1:300) with HEK-293 cells lysate 2000ug.

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  IHC staining of human gliomas using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11899-1-AP (TERF1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11899-1-AP (TERF1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 11899-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11899-1-AP (TERF1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Eierstockgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11899-1-AP bindet in WB, IHC, IF, IP, ELISA TERF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TERF1 fusion protein Ag2491
• Vollständiger Name: telomeric repeat binding factor (NIMA-interacting) 1
• Berechnetes Molekulargewicht: 439 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC029378
• Gene symbol: TERF1
• Gene ID (NCBI): 7013
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Telomeres are nucleoprotein complexes located at chromosome ends that consist of tandem arrays of TTAGGG repeats bound to specific proteins. Telomeres protect chromosome ends from degradation and end-to-end fusions, prevent activation of DNA damage checkpoints, and modulate the maintenance of telomeric DNA by telomerase. TERF1 (telomeric repeat-binding factor 1) binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. It's one component of the shelterin complex, which involved in the regulation of telomere length and protection.

Publikationen

Species	Application	Title
mouse	IF	PLoS Genet; IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.; Authors - Bo Chen
human	WB	CNS Neurosci Ther; FHOD1 is upregulated in glioma cells and attenuates ferroptosis of glioma cells by targeting HSPB1 signaling; Authors - Fan Zhang
human	WB	Sci Adv; Structural basis of telomeric nucleosome recognition by shelterin factor TRF1; Authors - Hongmiao Hu
rat	IHC	Eur J Pharmacol; Pentoxifylline protects against cerebral ischaemia-reperfusion injury through ferroptosis regulation via the Nrf2/SLC7A11/GPX4 signalling pathway; Authors - Pei Li
human	WB	J Proteome Res; Plasma Proteomics Analysis of Early Biomarkers for Predicting Female Fecundability: A Nested Case-Control Study; Authors - Lingling Huang