TER-119 Monoklonaler Antikörper

TER-119 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

TER-119

Kat-Nr. : CL647-65149

Synonyme

Ly76, lymphocyte antigen 76, TER 119, Ter119, TER-119

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Galerie der Validierungsdaten

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  FC experiment of mouse bone marrow cells using CL647-65149

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD45 (FITC-65087, Clone: 30-F11) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Mouse TER-119 (CL647-65149, Clone: TER-119). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL647-65149

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD45 (FITC-65087, Clone: 30-F11) and CoraLite® Plus 647-conjugated Rat IgG2b isotype control antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65149 bindet in FC TER-119 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fetale Leberzellen von Tag 14 aus einer C57BL/6-Maus
• Vollständiger Name: lymphocyte antigen 76
• Gene symbol: Ly76
• Gene ID (NCBI): 104231
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TER-119 is a mouse erythroid lineage-specific monoclonal antibody that reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activities (PMID: 1975515; 10848813). TER-119 recognizes a 52-kDa molecule on erythrocyte membranes (PMID: 10848813). TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself (PMID: 10848813).