TDRKH specific Polyklonaler Antikörper

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• KD/KO Validated

TDRKH specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 13528-1-AP

Synonyme

TDRKH, Tudor domain-containing protein 2, Tudor and KH domain-containing protein, tudor and KH domain containing, TDRD2

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Galerie der Validierungsdaten

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  WB analysis of NCCIT using 13528-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 13528-1-AP (TDRKH specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13528-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13528-1-AP (TDRKH specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13528-1-AP

  IP result of anti-TDRKH specific (IP:13528-1-AP, 4ug; Detection:13528-1-AP 1:7000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13528-1-AP (TDRKH specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13528-1-AP (TDRKH specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13528-1-AP (TDRKH specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13528-1-AP (TDRKH specific antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 13528-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13528-1-AP (TDRKH specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13528-1-AP

  Immunofluorescent analysis of Hela cells, using TDRKH antibody 13528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NCCIT cell, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 11 publications below
• IP: See 4 publications below
• RIP: See 1 publications below

Produktinformation

13528-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA TDRKH specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TDRKH specific fusion protein Ag4422
• Vollständiger Name: tudor and KH domain containing
• Berechnetes Molekulargewicht: 606 aa, 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC032690
• Gene symbol: TDRKH
• Gene ID (NCBI): 11022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

Publikationen

Species	Application	Title
mouse	WB	Nat Cell Biol; Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors.; Authors - Yu H Sun
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
mouse	IF, WB	Nat Commun; PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.; Authors - Deqiang Ding ; KO Validated
mouse	IP	EMBO J; MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA.; Authors - Chia-Ling Hsieh
mouse	WB,IF	EMBO J; Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline.; Authors - Jonathan P Saxe ; KO Validated
mouse	WB,IHC	Proc Natl Acad Sci U S A; Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi.; Authors - Chen Chen C