TCEB1 Polyklonaler Antikörper

TCEB1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12450-1-AP

Synonyme

SIII p15, Elongin-C, Elongin C, Elongin 15 kDa subunit, EloC

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Galerie der Validierungsdaten

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  WB analysis using 12450-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12450-1-AP (TCEB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12450-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12450-1-AP (TCEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12450-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12450-1-AP (TCEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12450-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12450-1-AP (TCEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12450-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12450-1-AP (TCEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12450-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TCEB1 antibody (12450-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushodengewebe, MCF-7-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12450-1-AP bindet in WB, IHC, IF/ICC, ELISA TCEB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCEB1 fusion protein Ag3087
• Vollständiger Name: transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
• Berechnetes Molekulargewicht: 112 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC013809
• Gene symbol: TCEB1
• Gene ID (NCBI): 6921
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCEB1, also named as RNA polymerase II transcription factor SIII subunit C, belongs to the SKP1 family. It mediates the ubiquitination of ECS-E3 ubiquitin-protein ligase complexes. TCEB1 is overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. TCEB1 also promotes invasion of prostate cancer cells (PMID:18844214).

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation.; Authors - Pingzhao Zhang
human	IHC	Am J Pathol; A 12-gene expression signature is associated with aggressive histological in prostate cancer: SEC14L1 and TCEB1 genes are potential markers of progression.; Authors - Agell Laia L
rat	WB	Evid Based Complement Alternat Med; Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism.; Authors - Chong-Chong Guo
human	WB	bioRxiv; The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation; Authors - Magda Wąchalska
human	WB,IF	Cell Death Differ; USP51 facilitates colorectal cancer stemness and chemoresistance by forming a positive feed-forward loop with HIF1A; Authors - Mingchao Mu