TBX6 Polyklonaler Antikörper

TBX6 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12447-1-AP

Synonyme

DFNB67, T box 6, T box protein 6, TBX6

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12447-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 12447-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12447-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12447-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12447-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12447-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12447-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 12447-1-AP

  IP result of anti-TBX6 (IP:12447-1-AP, 4ug; Detection:12447-1-AP 1:500) with human placenta tissue lysate 2800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, A549-Zellen, humanes Lungengewebe, humanes Plazenta-Gewebe, Mauslungengewebe, Raji-Zellen
• Erfolgreiche IP: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12447-1-AP bindet in WB, IP, ELISA TBX6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBX6 fusion protein Ag3050
• Vollständiger Name: T-box 6
• Berechnetes Molekulargewicht: 436 aa, 47 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC026031
• Gene symbol: TBX6
• Gene ID (NCBI): 6911
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBX6, also named as T-box transcription factor TBX6, is a 436 amino acid protein, which localizes in the nucleus and forms a dimeric complex with DNA. T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. The calculated molecular weight of TBX6 is 47 kDa, but the modified TBX6 is about 58 kDa.