TBX4/5 Polyklonaler Antikörper

TBX4/5 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 13178-1-AP

Synonyme

TBX5, T-box transcription factor TBX5, T-box protein 5, T box 5, HOS

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 13178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13178-1-AP (TBX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13178-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13178-1-AP (TBX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13178-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13178-1-AP (TBX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 13178-1-AP

  Immunofluorescent analysis of HepG2 cells using 13178-1-AP (TBX5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 3 publications below

Produktinformation

13178-1-AP bindet in WB, IF/ICC, ELISA TBX4/5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBX4/5 fusion protein Ag3845
• Vollständiger Name: T-box 5
• Berechnetes Molekulargewicht: 518 aa, 57 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC027942
• Gene symbol: TBX5
• Gene ID (NCBI): 6910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

T-box genes encode a family of transcription factors that have been identified in all metazoans and which play diverse roles during embryonic development. TBX5, a member of T-box family, has an essential role in the initiation of limb outgrowth and heart development. Genetic variation near TBX5 that associates with PR and/or QRS interval variation, which suggests that TBX5 plays a role in CCS function in the general population. Because 77 % amino acid sequence of TBX5 is identical to TBX4, this antibody recognizes both TBX4 and TBX5.

Publikationen

Species	Application	Title
human	IF	Eur Respir J; Single-cell RNA sequencing reveals that BMPR2 mutation regulates right ventricular function via ID genes.; Authors - Mingxia Du
human	WB	Mol Biol Evol; Functional and Adaptive Significance of Promoter Mutations that Affect Divergent Myocardial Expressions of TRIM72 in Primates.; Authors - Yuanqing Feng
mouse	WB	Environ Sci Technol; Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling.; Authors - Zhihua Ren
		Stem Cell Reports; Soft Matrix Promotes Cardiac Reprogramming via Inhibition of YAP/TAZ and Suppression of Fibroblast Signatures.; Authors - Shota Kurotsu
human	IF	iScience; A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells.; Authors - Zaniar Ghazizadeh
mouse	WB	Sci Rep; Stoichiometric optimization of Gata4, Hand2, Mef2c, and Tbx5 expression for contractile cardiomyocyte reprogramming.; Authors - Zhentao Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Young-Jae (Verified Customer) (12-06-2018)

Working good for western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: fibroblasts overexpressed with Tbx5