TBX21,T-bet Polyklonaler Antikörper

TBX21,T-bet Polyklonal Antikörper für WB, IP, IHC, ELISA, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA, FC (Intra)

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 13700-1-AP

Synonyme

T bet, T box 21, T box protein 21, T PET, TBET, TBLYM, TBX21, TBX21,T bet, TBX21,T-bet, Transcription factor TBLYM

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Galerie der Validierungsdaten

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  WB analysis of A549 using 13700-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13700-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21,T-bet Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 13700-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 13700-1-AP

  IP result of anti-TBX21,T-bet (IP:13700-1-AP, 4ug; Detection:13700-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human tonsillitis using 13700-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 13700-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of NK92 using 13700-1-AP

  1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human TBX21,T-bet (13700-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Maus-Thymusgewebe, RAW 264.7-Zellen
• Erfolgreiche IP: Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): NK92
• Erfolgreiche Detektion in FC: NK92

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

13700-1-AP bindet in WB, IP, IF, IHC, ELISA, FC (Intra) TBX21,T-bet und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBX21,T-bet fusion protein Ag4618
• Vollständiger Name: T-box 21
• Berechnetes Molekulargewicht: 535 aa, 58 kDa
• Beobachtetes Molekulargewicht: 60-62 kDa
• GenBank-Zugangsnummer: BC039739
• Gene symbol: TBX21/T-bet
• Gene ID (NCBI): 30009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.

Publikationen

Species	Application	Title
human	WB	Cell; Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.; Authors - Rui Yang ; KD Validated
rat	WB	Int Immunopharmacol; Modulating Th1/Th2 drift in asthma-related immune inflammation by enhancing bone mesenchymal stem cell homing through targeted inhibition of the Notch1/Jagged1 signaling pathway; Authors - Wang Kun
human	WB	Int J Cardiol; Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome.; Authors - Lu Yongguang Y
mouse	WB	Mol Nutr Food Res; A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways; Authors - Qianye Zhang
rat	WB	Biomed Pharmacother; Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.; Authors - Yu Xia
human	WB	Front Pharmacol; Targeting Wnt Signaling in the Tumor Immune Microenvironment to Enhancing EpCAM CAR T-Cell therapy.; Authors - Weizhen Li