TBL2 Polyklonaler Antikörper

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• KD/KO Validated

TBL2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 12488-1-AP

Synonyme

Williams-Beuren syndrome chromosomal region 13 protein, WBSCR13, UNQ563/PRO1125, Transducin beta-like protein 2, Transducin beta like protein 2

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  WB analysis of COLO 320 using 12488-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12488-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 12488-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 12488-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12488-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12488-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12488-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12488-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12488-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 12488-1-AP

  IP result of anti-TBL2 (IP:12488-1-AP, 4ug; Detection:12488-1-AP 1:800) with COLO 320 cells lysate 2400ug.

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  IHC staining of human pancreas cancer using 12488-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12488-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12488-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 12488-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 12488-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12488-1-AP

  Immunofluorescent analysis of () fixed HepG2 cells using 12488-1-AP (TBL2 antibody) at dilution of 1:25.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, Maus-Pankreasgewebe, Maus-Plazenta-Gewebe
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Herzgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

12488-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TBL2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBL2 fusion protein Ag3200
• Vollständiger Name: transducin (beta)-like 2
• Berechnetes Molekulargewicht: 446 aa, 50 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC012938
• Gene symbol: TBL2
• Gene ID (NCBI): 26608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.

Publikationen

Species	Application	Title
human	WB,IHC	Adv Sci (Weinh); TBL2 Promotes Tumorigenesis via PRMT5/WDR77-Mediated AKT Activation in Breast Cancer; Authors - Xiuqing Lu
	WB,IF	Adv Sci (Weinh); Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia; Authors - Ningjing Ou
human	WB,IF	PLoS One; TBL2 Is a Novel PERK-Binding Protein that Modulates Stress-Signaling and Cell Survival during Endoplasmic Reticulum Stress.; Authors - Yoshinori Tsukumo ; KD Validated
	WB	J Cell Biochem; TBL2 Associates With ATF4 mRNA Via Its WD40 Domain and Regulates Its Translation During ER Stress.; Authors - Yoshinori Tsukumo
human	WB, IF	Biochem Biophys Res Commun; The endoplasmic reticulum-localized protein TBL2 interacts with the 60S ribosomal subunit.; Authors - Yoshinori Tsukumo
human	WB,IHC	Ann Surg Oncol; Transducin Beta-Like 2 is a Potential Driver Gene that Adapts to Endoplasmic Reticulum Stress to Promote Tumor Growth of Lung Adenocarcinoma; Authors - Keisuke Kosai ; KD Validated