TAX1BP3 Polyklonaler Antikörper

TAX1BP3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (4)

Kat-Nr. : 11692-1-AP

Synonyme

Tax interaction protein 1, Tax1 binding protein 3, TAX1BP3, TIP 1, TIP1

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Galerie der Validierungsdaten

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  WB analysis using 11692-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11692-1-AP (TAX1BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mausnierengewebe, NIH/3T3-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

11692-1-AP bindet in WB, IHC, ELISA TAX1BP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAX1BP3 fusion protein Ag2306
• Vollständiger Name: Tax1 (human T-cell leukemia virus type I) binding protein 3
• Berechnetes Molekulargewicht: 124 aa, 14 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC023980
• Gene symbol: TAX1BP3
• Gene ID (NCBI): 30851
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tax-interacting protein 1 (TIP-1, also known as Tax1bp3 or glutaminase-interacting protein, GIP) is unique in its simple structure, a single PDZ domain is the only functional and structural unit identified so far in this small PDZ protein (124 amino acids in human and mouse). High level of TIP-1 expression was detected in human invasive breast cancer cells and that is important for tumor cell adhesion, migration and pulmonary metastasis. However, the precise biological effects of TIP-1 and its functional mechanisms have largely remained elusive. Mutation of TAX1BP3 can cause Dilated Cardiomyopathy with Septo-Optic Dysplasia (PMID: 25645515).

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Gene; Birc3 and Tip1 are upregulated in renal ischemia reperfusion injury; Authors - Sixu Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Good clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,

FH

Jie (Verified Customer) (01-23-2020)

works well with a specific band that matches the product size

• Applications: Western Blot,
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Human kidney biopsy

FH

Canzhao (Verified Customer) (01-17-2020)

It is one of the best antibody I used. It is very specific. I would like to give it 6 stars if it is possible.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
• Cell Tissue Type: Mouse heart and cultured cardiomyocytes.